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maltosebinding protein mbp  (New England Biolabs)


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    Structured Review

    New England Biolabs maltosebinding protein mbp
    Maltosebinding Protein Mbp, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/maltosebinding+protein+mbp/pm12582727-71-9-26
    Average 86 stars, based on 1 article reviews
    maltosebinding protein mbp - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Fluorescence:

    Article Title: Identification of Common Binding Sites for Calmodulin and Inositol 1,4,5-Trisphosphate Receptors on the Carboxyl Termini of Trp Channels
    Article Snippet: By design, the insert in each fusion protein starts with a Met and ends with a “TAA” stop codon (T comes from the A in the antisense primer, while AA comes from the pCRII vector). .. Complementary DNA for enhanced blue fluorescence protein (EBFP) was excised from pEBFP-C1 (CLONTECH) and subcloned into pAGA (29) at NcoI/SmaI sites, while that for maltosebinding protein (MBP) was amplified from pMAL-C2 (New England Biolabs) by PCR, excised with MfeI/NcoI, and subcloned into pAGA at EcoRI/NcoI sites. ..

    Amplification:

    Article Title: Identification of Common Binding Sites for Calmodulin and Inositol 1,4,5-Trisphosphate Receptors on the Carboxyl Termini of Trp Channels
    Article Snippet: By design, the insert in each fusion protein starts with a Met and ends with a “TAA” stop codon (T comes from the A in the antisense primer, while AA comes from the pCRII vector). .. Complementary DNA for enhanced blue fluorescence protein (EBFP) was excised from pEBFP-C1 (CLONTECH) and subcloned into pAGA (29) at NcoI/SmaI sites, while that for maltosebinding protein (MBP) was amplified from pMAL-C2 (New England Biolabs) by PCR, excised with MfeI/NcoI, and subcloned into pAGA at EcoRI/NcoI sites. ..

    Polymerase Chain Reaction:

    Article Title: Identification of Common Binding Sites for Calmodulin and Inositol 1,4,5-Trisphosphate Receptors on the Carboxyl Termini of Trp Channels
    Article Snippet: By design, the insert in each fusion protein starts with a Met and ends with a “TAA” stop codon (T comes from the A in the antisense primer, while AA comes from the pCRII vector). .. Complementary DNA for enhanced blue fluorescence protein (EBFP) was excised from pEBFP-C1 (CLONTECH) and subcloned into pAGA (29) at NcoI/SmaI sites, while that for maltosebinding protein (MBP) was amplified from pMAL-C2 (New England Biolabs) by PCR, excised with MfeI/NcoI, and subcloned into pAGA at EcoRI/NcoI sites. ..

    Article Title: Characterization of the expression and immunogenicity of the ns4b protein of human coronavirus 229E
    Article Snippet: Sequencing of complementary DNAs prepared from various coronaviruses has revealed open reading frames encoding putative proteins that are yet to be characterized and are so far only described as nonstructural (ns).. As a first step in the elucidation of its function, we characterized the expression and immunogenicity of the ns4b gene product from strain 229E of human coronavirus (HCV-229E), a respiratory virus with a neurotropic potential.. The gene was cloned and expressed in bacteria.

    Construct:

    Article Title: Characterization of a putative fusogen encoded in a mitochondrial plasmid of Physarum polycephalum.
    Article Snippet: The mitochondrial plasmid mF induces mitochondrial fusion in zygotes and during sporulation in the true slime mold Physarum polycephalum .. There are nine open reading frames (ORFs) in the mF plasmid, and it has been suggested that ORF640 encodes the mitochondrial fusogen.. We prepared antisera directed against the ORF640 protein (ORF640p) in rabbits, and used it to localize this protein in mitochondria.

    Article Title: Indoleacetate decarboxylase is a glycyl radical enzyme catalysing the formation of malodorant skatole.
    Article Snippet: Codon-optimized gene fragments of IAD and IADAE were synthesized by General Biosystems, Inc. and were inserted into vectors pET-28a-HT and pET-28a-HMT, respectively. .. The former contained a His6-tag and a Tobacco Etch Virus (TEV) protease cleavage site, followed by the construct of interest while the latter contained, in tandem, a His6-tag, maltosebinding protein (MBP) and a TEV protease cleavage site, followed by the construct of interest40 both at the SspI restriction sites, using the Gibson Assembly® Cloning protocol (New England Biolabs, Ipwich, MA, USA). .. For IAD expression, E. coli BL21 (DE3) cells (New England Biolabs, Ipwich, MA, USA) were transformed with the plasmid HT-IAD, and grown in LB supplemented with 50 μg/mL kanamycin.

    Cloning:

    Article Title: Characterization of a putative fusogen encoded in a mitochondrial plasmid of Physarum polycephalum.
    Article Snippet: The mitochondrial plasmid mF induces mitochondrial fusion in zygotes and during sporulation in the true slime mold Physarum polycephalum .. There are nine open reading frames (ORFs) in the mF plasmid, and it has been suggested that ORF640 encodes the mitochondrial fusogen.. We prepared antisera directed against the ORF640 protein (ORF640p) in rabbits, and used it to localize this protein in mitochondria.

    Article Title: Characterization of the expression and immunogenicity of the ns4b protein of human coronavirus 229E
    Article Snippet: Sequencing of complementary DNAs prepared from various coronaviruses has revealed open reading frames encoding putative proteins that are yet to be characterized and are so far only described as nonstructural (ns).. As a first step in the elucidation of its function, we characterized the expression and immunogenicity of the ns4b gene product from strain 229E of human coronavirus (HCV-229E), a respiratory virus with a neurotropic potential.. The gene was cloned and expressed in bacteria.

    Article Title: Indoleacetate decarboxylase is a glycyl radical enzyme catalysing the formation of malodorant skatole.
    Article Snippet: Codon-optimized gene fragments of IAD and IADAE were synthesized by General Biosystems, Inc. and were inserted into vectors pET-28a-HT and pET-28a-HMT, respectively. .. The former contained a His6-tag and a Tobacco Etch Virus (TEV) protease cleavage site, followed by the construct of interest while the latter contained, in tandem, a His6-tag, maltosebinding protein (MBP) and a TEV protease cleavage site, followed by the construct of interest40 both at the SspI restriction sites, using the Gibson Assembly® Cloning protocol (New England Biolabs, Ipwich, MA, USA). .. For IAD expression, E. coli BL21 (DE3) cells (New England Biolabs, Ipwich, MA, USA) were transformed with the plasmid HT-IAD, and grown in LB supplemented with 50 μg/mL kanamycin.

    Reverse Transcription:

    Article Title: Characterization of the expression and immunogenicity of the ns4b protein of human coronavirus 229E
    Article Snippet: Sequencing of complementary DNAs prepared from various coronaviruses has revealed open reading frames encoding putative proteins that are yet to be characterized and are so far only described as nonstructural (ns).. As a first step in the elucidation of its function, we characterized the expression and immunogenicity of the ns4b gene product from strain 229E of human coronavirus (HCV-229E), a respiratory virus with a neurotropic potential.. The gene was cloned and expressed in bacteria.

    Binding Assay:

    Article Title: CRISPR/Cas9-Induced Mutagenesis Corroborates the Role of Transportin-SR2 in HIV-1 Nuclear Import
    Article Snippet: The frozen cultures were thawed, resuspended in lysis buffer (50 mM Tris-HCl [pH 7.5], 350 mM NaCl, 2 units DNase/10 ml, and 1 tablet Complete protease inhibitor/50 ml [Roche]) and lysed by sonication, and the lysate was centrifuged at 15,000 rpm at 4°C for 30 min. .. The soluble lysate containing maltosebinding protein (MBP)-fused protein was loaded onto an amylose resin column (New England BioLabs) equilibrated with binding buffer (50 mM Tris-HCl [pH 7.5], 350 mM NaCl). ..

    Virus:

    Article Title: Indoleacetate decarboxylase is a glycyl radical enzyme catalysing the formation of malodorant skatole.
    Article Snippet: Codon-optimized gene fragments of IAD and IADAE were synthesized by General Biosystems, Inc. and were inserted into vectors pET-28a-HT and pET-28a-HMT, respectively. .. The former contained a His6-tag and a Tobacco Etch Virus (TEV) protease cleavage site, followed by the construct of interest while the latter contained, in tandem, a His6-tag, maltosebinding protein (MBP) and a TEV protease cleavage site, followed by the construct of interest40 both at the SspI restriction sites, using the Gibson Assembly® Cloning protocol (New England Biolabs, Ipwich, MA, USA). .. For IAD expression, E. coli BL21 (DE3) cells (New England Biolabs, Ipwich, MA, USA) were transformed with the plasmid HT-IAD, and grown in LB supplemented with 50 μg/mL kanamycin.

    Expressing:

    Article Title: Arabidopsis RNA-binding Protein FCA Regulates MicroRNA172 Processing in Thermosensory Flowering
    Article Snippet: .. Preparation ofMBP-FCAFusion Protein—The FCA genewas subcloned into the pMAL-c2X Escherichia coli expression vector (New England Biolabs, Ipswich, MA) containing a maltosebinding protein (MBP)-coding sequence. .. Recombinant MBP andMBP-FCAproteins were synthesized in the E. coliRosetta2 (DE3) pLysS strain (Novagen, Madison,WI) and partially purified as described previously (30).

    Sequencing:

    Article Title: Arabidopsis RNA-binding Protein FCA Regulates MicroRNA172 Processing in Thermosensory Flowering
    Article Snippet: .. Preparation ofMBP-FCAFusion Protein—The FCA genewas subcloned into the pMAL-c2X Escherichia coli expression vector (New England Biolabs, Ipswich, MA) containing a maltosebinding protein (MBP)-coding sequence. .. Recombinant MBP andMBP-FCAproteins were synthesized in the E. coliRosetta2 (DE3) pLysS strain (Novagen, Madison,WI) and partially purified as described previously (30).



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